Research Suitability: Comparative organic solvent extraction of alkaloidal constituents – compare alkaloids extracted using different solvents as well as alkaloid content with different kratom varieties. Component separation by High Pressure Liquid Chromatography or Thin Layer Chromatography. Differential separation, identification and isolation of known compounds for comparison with either different Mitragyna spp. or other closely related taxa. Molecular characterization and identification of isolated constituents by IR spectroscopy and/or Nuclear Magnetic Resonance – analytical chemistry. In-Vitro studies of effects on isolated or cultured tissues (eg. Pig ileum cells) or binding assays on isolated neuronal receptors to determine putative physiological effects. Examination of subtrate-ligand binding mechanisms, affinities and dissociation constants (Kd). Not intended or authorized for in-vivo veterinary use. Suitable for use as botanical specimen for collections.
Research Notes: The new, micro-grind enables efficient solvent extraction for chromatographic analysis by increasing the availability of compounds for separation and elution.